show Abstracthide AbstractIt remains elusive whether the plants occurring in the Qinghai-Tibetan Plateau and western desert regoin shared the common responses to the climatic changes and environmental heterogeneity since Quaternary. Then microclimatic caused by landscape changes and human activities could also promoted intra and interspecific divergence. In the present project, we selected Caragana jubata and Caragana roborovskyi to compare the genetic diversity and climate adaptation inter- and intraspecies, which based on GBS analysis. As closely related Eurychoric Caragana species-pairs, they respectively adapted in the Qinhai-Tibetan plateau alpine steppe and become constructive species in the desert region of Northwest China. It seemed that early origin but differentiated gradually influenced by the microenvironments due to the qinghai-tibet plateau uplift. We will apply genotyping by sequencing (GBS) to detect genetic diversity, gene flow, and selection of individuals between and within populations, firstly compare phylogenetic tree and analysis phylogeographic structures, which build on neutral and non-neutral SNP pairs; secondly, clarify all historical changes and evolutionary process that resulted in the current distributions of two species. It will be a good case of studying patterns of genomic divergence response to climatic changes, and may shed light on the underlying evolutionary processes: Since Quaternary till now, the alpine and desert Caragana species share the same pattern of genetic diversity, or not? Such detailed comparisons will effectively reveal the adaptation mechimanism of these two plants, and also for further conservation and unitilization of these Caragana species resources.